Initial Fertility Testing (AFAB & AMAB)

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A micrograph of In Vitro Fertilization.

Preliminary Testing at TRIO

Welcome to TRIO Fertility, and thank you for taking the first step in your fertility journey with us. After your initial appointment, your physician has recommended specific tests to help us build a clear and personalized plan for you. Below, you’ll find everything you need to know about these tests, including instructions, timing, and next steps.

As your doctor mentioned, testing is based around the timing of your cycle. Day 1 is the first day of your period, full flow – not spotting.

Please email both the Day 1 team (day1@triofertility.com), along with cc’ing your doctor’s office on the first day of your next period, and we will help to arrange the following tests at TRIO:

Female or Assigned Female at Birth (AFAB) Fertility Testing

  • Viral Bloodwork (Infectious Diseases)
  • AMH blood work. Please note that this test is not covered by OHIP. The fee is $125.
  • If a Vitamin D test is requested, it is $51 and billed directly through LifeLabs, 
  • Cycle day 3-5 blood work.
  • Cycle day 3-5 full bladder pelvic ultrasound.
  • Cycle day 5-12 Sonohysterogram (learn what to expect at your sonohysterogram) 
    • Please start your antibiotics prior to your sonohysterogram as prescribed by your TRIO physician. 
    • Important Reminder: Please abstain from intercourse (or use protection) from Day 1 of your reported cycle until AFTER your sonohysterogram has been completed.

Male or Assigned Male at Birth (AMAB) Fertility Testing

  • Viral bloodwork (Infectious Diseases)
  • DNA Fragmentation Analysis or Semen Analysis. The fees for the DNA Fragmentation test is $350.
  • Your booking instructions will be on the requisition.
  • Review the instructions for sperm testing here.  

Questions? 

If you have any questions during your testing phase, please contact your doctor’s administrative team directly, as they are best equipped to support you and coordinate next steps in your care. Our team is here to support you—please don’t hesitate to reach out to the appropriate person below as you complete your testing.

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Administrative Contacts

Frequently Asked Questions

Fertility testing helps your TRIO care team understand your baseline fertility profile so your physician can recommend the most appropriate next steps and create a tailored plan. Learn more:  https://triofertility.com/intro-fertility/

Common testing may include bloodwork (hormone testing), ultrasound (to assess ovaries and follicles), and—when applicable—sperm testing via semen analysis. Your physician will confirm which tests apply to you.

Some blood tests are typically completed early in the menstrual cycle (between days 2-5) to assess hormone levels that relate to egg supply and ovulation. Other testing can be done at any time. Follow the timing provided by your care team.

In some cases, fasting bloodwork may be required. If fasting is required your doctor will let you know. 

Some new patient testing requires a urine sample. You’ll be asked to arrive early to collect a specimen jar from the blood lab before proceeding to your ultrasound appointment.

Your ultrasound will include two steps:

  1. a full bladder transabdominal ultrasound, followed by
  2. a transvaginal ultrasound after you empty your bladder.

If you are doing your testing at a TRIO location we will have your bloodwork requisitions on file. If you are doing bloodwork at an outside lab you will need to bring all requisitions with you to ensure all tests are completed. You also may be asked for a copy of your ultrasound requisition, which your medical team will email to you.

A sonohysterogram is a diagnostic procedure used to evaluate the uterine cavity and may also help assess whether the fallopian tubes are open. If scheduled, you may be advised to take pain relief (e.g., Advil or Tylenol) about one hour before. You will also be given antibiotic with instructions to start it before the procedure and complete the prescribed course.

You must abstain from unprotected intercourse after your cycle begins until the sonohysterogram is completed.

A semen analysis is commonly used to evaluate sperm number, movement, and shape to help rule out sperm-related factors that may affect conception.A DNA fragmentation test is a specialized laboratory analysis that measures the percentage of sperm cells with damaged or broken DNA strands, evaluating genetic quality beyond a standard semen analysis. Both tests can be done to identify causes of unexplained infertility, recurrent pregnancy loss, and failed IVF/ICSI cycles, helping clinicians choose the best treatment path.

During the testing phase, please direct questions to your physician’s administrative team (contact details listed on this page). This helps ensure the right team can coordinate your testing, answer process questions, and support next steps efficiently.

TRIO Owned & Operated Locations

TRIO Fertility (Main Location)

655 Bay Street
Toronto, Ontario M5G 2K4
11th and 18th floors

Hours of Operation

Monday 7:00 a.m. to 4:00 p.m.
Tuesday 7:00 a.m. to 4:00 p.m.
Wednesday 7:00 a.m. to 4:00 p.m.
Thursday 7:00 a.m. to 4:00 p.m.
Friday 7:00 a.m. to 4:00 p.m.
Saturday 7:00 a.m. to 1:00 p.m.
Sunday 7:00 a.m. to 1:00 p.m.

Phone

Fax

416-506-0600

Learn More

Thornhill @ TRIO Clinic

7330 Yonge Street, Suite 217
Thornhill, Ontario L4J 7Y7

Hours of Operation

Monday 7:00 a.m. to 3:00 p.m.
Tuesday 7:00 a.m. to 3:00 p.m.
Wednesday 7:00 a.m. to 3:00 p.m.
Thursday 7:00 a.m. to 3:00 p.m.
Friday 7:00 a.m. to 3:00 p.m.

Phone

Learn More

North York @ TRIO Clinic

Hours of Operation

36 York Mills Rd., Suite 100.
North York, ON M2P 2E9
Monday 7:00 a.m. to 3:00 p.m.
Tuesday 7:00 a.m. to 3:00 p.m.
Wednesday 7:00 a.m. to 3:00 p.m.
Thursday 7:00 a.m. to 3:00 p.m.
Friday 7:00 a.m. to 3:00 p.m.

Phone

Website

Burlington @ TRIO Clinic

Hours of Operation

3115 Harvester Rd., Suite 301
Burlington, ON L7N 3N8
Monday 7:00 a.m. to 3:00 p.m.
Tuesday 7:00 a.m. to 3:00 p.m.
Wednesday 7:00 a.m. to 3:00 p.m.
Thursday 7:00 a.m. to 3:00 p.m.
Friday 7:00 a.m. to 3:00 p.m.

Phone

Website